| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tri-Cities United Soccer Club Tri-Cities United 08/09 Boys Premier |
0106-012B3U-0004 | Izudin Operta |
| A2 | Tennessee Soccer Club TSC Murfreesboro 2009 Boys Showcase |
0522-013B3U-2007 | Josh Inglis |
| A3 | FC Academy FC Academy - 0407 - B13U |
0407-012B3U-1234 | Luis Altamirano |
| A4 | Chattanooga FC Academy Chattanooga FC Academy 2008 White Boys |
0316-022B3U-7792 | Gary Smith |
| A5 | International Soccer Club International Soccer Club - 08elite |
0536-012B3U-2007 | Ruben Arcega |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B13U Red |
0521-012B3U-2050 | Tommy Holt |
| A7 | Tennessee Soccer Club TSC Nashville 2008 Boys Showcase |
0522-012B3U-0078 | Coy Boazman |
| A8 | Tennessee United Soccer Club TUSC 2008 Boys Blue |
0424-012B3U-0018 | Bryson Lehn |