| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Knoxville FC (Crush) Knoxville FC Crush 07B Premier |
0206-011B4U-0040 | Fabio Suarez |
| A2 | Tennessee Soccer Club TSC 2007 Boys ECNL |
0522-011B4U-0081 | Gregory Warden |
| A3 | Lobos Rush (Collierville Soccer Association) 07 Lobos Rush Memphis Blues |
0603-011B4U-0002 | Russell Church |
| A4 | Lobos Rush (Collierville Soccer Association) 2007 Lobos Rush Premier |
0603-011B4U-0032 | David McAlister |
| A5 | Aris FC Aris FC - B14U Black 2007 |
0530-022B4U-0104 | Brian Davies |
| A6 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 07B SL |
0324-021B4U-0009 | Christopher Zeller |
| A7 | Memphis Futbol Club / Memphis Soccer Education Memphis FC/Ole 07 Boys Premier - SL |
0624-011B4U-2336 | William Pryor |
| A8 | Nashville United Soccer Academy - 01 NUSA B07 Maroon |
0534-011B4U-0008 | Giles Cheevers |
| A9 | Tennessee Soccer Club TSC 2007 Boys Nike |
0522-011B4U-0080 | Jordan Grant |
| A10 | Tennessee United Soccer Club TUSC 2007 Boys Red |
0424-011B4U-0032 | Eduardo Carvacho |