| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Clarksville Soccer Club Clarksville SC - B2007 Maroon |
0422-012B4U-0011 | Steve Young |
| A2 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2007B Garnet |
0500-01D2B4U-0027 | Eduardo Caturla |
| A3 | Jackson Soccer Club JSC 2007 Boys |
0609-012B4U-0157 | Malang Jarju |
| A4 | Germantown Legends Germantown Legends 07 Black |
0606-012B4U-0012 | Kirk Dunaway |
| A5 | Tennessee Soccer Club TSC Williamson 2007 Boys Showcase |
0522-012B4U-0079 | Tre Arhagba |
| A6 | Tennessee Soccer Club TSC Sumner 2007 Boys Showcase |
0522-012B4U-2013 | Travis Pulley |
| A7 | Nashville United Soccer Academy - 01 NUSA B07 Navy |
0534-012B4U-0019 | Alija Turkovic |