| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2009 Lady Lobos Rush Blue |
0603-01SG2U-0006 | John Cooke |
| A2 | Kings Hammer 2009 Lady Strikers Showcase |
0515-01DG2U-0020 | Lauren Mayer |
| A3 | Knoxville Reds Football Club - 01 Knoxville Reds 09 Ajax |
0290-01SJG2U-0004 | Derrick Long |
| A4 | Nashville United Soccer Academy - 01 NUSA G09 Maroon |
0534-01AG2U-0118 | Wade Wonderlin |
| A5 | Tennessee Soccer Club TSC 2009 Girls Showcase White |
0522-01AG2U-0104 | Jenger Parrish |
| A6 | Tennessee United Soccer Club TUSC 2009 Girls Red |
0424-01AG2U-0004 | Andrew Fish |