| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Soccer Club TSC 2008 Girls Nike |
0522-011G3U-0054 | Lance Mayes |
| A3 | Lobos Rush (Collierville Soccer Association) 2008 Lady Lobos Rush Premier |
0603-011G3U-0020 | Candice Spiniolas |
| A4 | Knoxville Reds Football Club - 01 Knoxville Reds 08 Newcastle |
0290-01D1G3U-0001 | Justin Lloyd |
| A5 | Nashville United Soccer Academy - 01 NUSA G08 Maroon |
0534-011G3U-0001 | Adam Harris |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - G13U Black |
0521-011G3U-2051 | Jamonn Brady |
| A7 | Tennessee Soccer Club TSC 2008 Girls ECNL |
0522-011G3U-0055 | Gregory Warden |
| A8 | USA North USA North 08G TSL |
0218-01D1G3U-0402 | Victoria Weinhandl |