| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2008 Girls Blue |
0424-012G3U-0024 | David Pepper |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2008 Navy Girls |
0316-02DG3U-7807 | Stephen Hirayama |
| A3 | Knoxville FC (Crush) Knoxville FC Crush 2008G Premier |
0206-012G3U-0035 | Lindsay Taylor |
| A4 | Wilson United Soccer Club EESC 2008 Girls |
0406-012G3U-1037 | Mike Moore |
| A5 | Tri-Cities United Soccer Club Tri-Cities United 08 Girls Premier |
0106-012G3U-0005 | Leah Presley |