| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2008G Red |
0500-01D3G3U-0029 | James Branch |
| A2 | Blount United Soccer Club Blount United Soccer Club -- G13U |
0201-013G3U-0001 | Franco Zermeno |
| A3 | Chattanooga FC Academy Chattanooga FC Academy 2008 White Girls |
0316-024G3U-7808 | Daniel Silva |
| A4 | Gallatin Rangers GSC Lady Rangers 08 |
0408-023G3U-0034 | Michael Thomas |
| A5 | Knoxville FC (Crush) Knoxville FC Crush 2008G Stoke City |
0206-013G3U-0134 | Maggie Mamantov |