| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Wilson United Soccer Club EESC 2007 Girls |
0406-011G4U-1030 | Monica Ellmore |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 07G SL |
0324-021G4U-0006 | Luke Winter |
| A3 | Knoxville Reds Football Club - 01 Knoxville Reds 07 Breakers |
0290-01D1G4U-0007 | Dexter Cox |
| A4 | Tennessee Soccer Club TSC 2007 Girls ECNL |
0522-011G4U-0058 | Kevin O'Brien |
| A5 | Tennessee Soccer Club TSC 2007 Girls Nike |
0522-011G4U-0057 | Stephen Jordan |
| A6 | Tennessee United Soccer Club TUSC 2007 Girls Red |
0424-011G4U-0014 | A Morrell |