| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy 2007 Navy Girls |
0316-02DG4U-7809 | Ron Senowech |
| A2 | USA North USA North 07G Premier |
0218-01D2G4U-0371 | Darrick Lubell |
| A3 | Gallatin Rangers GSC Lady Rangers 07 |
0408-022G4U-0035 | Charles Shinton |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 07G Premier |
0206-012G4U-0034 | Kristen Lyons |
| A5 | Tennessee United Soccer Club TUSC 2007 Girls Blue |
0424-012G4U-0025 | Nate Sego |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - G14U Red |
0521-012G4U-2052 | Charles Bozman |
| A7 | Tennessee Soccer Club TSC Murfreesboro 2007 Girls Showcase |
0522-012G4U-0056 | Jason Finley |