| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy 2006 Navy Girls |
0316-02GG5U-7810 | Juan Hernandez |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 06G SL |
0324-021G5U-0013 | Luke Winter |
| A3 | Tennessee Soccer Club TSC 2006 Girls Nike |
0522-011G5U-0062 | Jason Mazuk |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2006 Girls Showcase |
0522-011G5U-0059 | B Lewis |
| A5 | Germantown Legends Germantown Lady Legends 06 |
0606-011G5U-0002 | Traci Sipniewski |
| A6 | Knoxville Reds Football Club - 01 Knoxville Reds 06 Sevilla |
0290-01D1G5U-0010 | n/a |
| A7 | Nashville United Soccer Academy - 01 NUSA G06 Maroon |
0534-011G5U-0003 | Dana Weymouth |
| A8 | Tennessee United Soccer Club TUSC 2006 Girls Red |
0424-011G5U-0015 | Troy Dusosky |