| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Soccer Club TSC Murfreesboro 2006 Girls Premier |
0522-012G5U-0113 | Jorge Suarez |
| A2 | Tennessee Soccer Club TSC Williamson 2006 Girls Showcase |
0522-012G5U-0061 | Valerie Tysinger |
| A3 | Lobos Rush (Collierville Soccer Association) 06 Lady Lobos Rush Elite |
0603-012G5U-0044 | Jason Haluska |
| A4 | Arlington Soccer Academy - 01 Arlington Soccer Academy - 01 - G15U |
0627-012G5U-0055 | Ryan Paule |
| A5 | Clarksville Soccer Club Clarksville SC - G2006 Maroon |
0422-012G5U-0020 | Wayne Hibbeler |
| A6 | Jackson Soccer Club JSC 2006 Girls |
0609-01DG5U-0161 | Malang Jarju |
| A7 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 06/07 Girls Blue - SL |
0624-012G5U-2375 | Thomas Davis |
| A8 | Nashville United Soccer Academy - 01 NUSA G06 Navy |
0534-012G5U-0014 | Sydney Eddy |