| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Soccer Club TSC Murfreesboro 2005 Girls Showcase |
0522-011G6U-0064 | Christopher Louk |
| A2 | Tennessee Soccer Club TSC Williamson 2005 Girls Showcase |
0522-011G6U-0066 | Anthony Torres |
| A3 | Tennessee United Soccer Club TUSC 2005 Girls Red |
0424-011G6U-0016 | Nathanael Hubert |
| A4 | Soccer Ole Memphis FC/Soccer Ole 2005 girls premier |
0632-021G6U-0001 | Robby Midyett |
| A5 | Nashville United Soccer Academy - 01 NUSA G05 Maroon |
0534-011G6U-0004 | Deniz Ismailovic |
| A6 | Tennessee Soccer Club TSC Nashville 2005 Girls Showcase |
0522-011G6U-0065 | Sarah Richardson |