| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Knoxville FC (Crush) Knoxville FC Crush 05G Premier |
0206-012G6U-0120 | Stephen Lyons |
| A2 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - G16U Black |
0521-011G6U-2053 | Thomas Williams |
| A3 | USA North USA North 05G Premier |
0218-01D2G6U-0372 | Tim McMahan_Dup |
| A4 | All-In FC TN All-In FC TN 05 Girls |
0443-01SLG6U-0065 | Bralen Clouse |
| A5 | USA Southeast USA Southeast 2005 Girls Premier |
0218-04D2G6U-0003 | Josh Crispin |
| A6 | Camp Forest Football Club CFFC 05 GIRLS |
0523-022G6U-2088 | Andy Lyon |
| A7 | Knoxville Reds Football Club - 01 Knoxville Reds 05 Fire |
0290-01D2G6U-0012 | Gregory Huddy |