| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Soccer Club TSC Nashville 2005 Girls Premier |
0522-013G6U-0158 | Michael Alley |
| A2 | Nashville United Soccer Academy - 01 NUSA G05 Gold |
0534-013G6U-0120 | Sherwin Farhoudi |
| A3 | Nashville United Soccer Academy - 01 NUSA G05 Navy |
0534-013G6U-0015 | Sunebari Ndam |
| A4 | Soccer Ole Memphis FC/Soccer Ole 2005 girls elite |
0632-022G6U-0002 | Andres Flores |
| A5 | Columbia Soccer Association - 01 Columbia Arsenal U16G |
0504-013G6U-0151 | John Spencer |