| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Knoxville FC (Crush) Knoxville FC Crush 04G Premier |
0206-011G7U-0002 | David Baisden |
| A2 | Knoxville Reds Football Club - 01 Knoxville Reds 04 United |
0290-01D1G7U-0013 | Dexter Cox |
| A3 | Tennessee Soccer Club TSC Sumner 2004 Girls Showcase |
0522-011G7U-0068 | Travis Pulley |
| A4 | Nashville United Soccer Academy - 01 NUSA G04 Maroon |
0534-011G7U-0005 | Shane Keely |
| A5 | Tri-Cities United Soccer Club Tri-Cities United 04/05 Girls Premier |
0106-011G7U-0014 | Jordan Campbell |
| A6 | Tennessee Soccer Club TSC Murfreesboro 2004 Girls Showcase |
0522-011G7U-0069 | Jorge Suarez |