| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Camp Forest Football Club CFFC 03 GIRLS |
0523-022G8U-2086 | Katie Reid |
| A2 | Blount United Soccer Club Blount United Soccer Club - G18U |
0201-012G8U-0244 | Thomas McCallum |
| A3 | Wilson United Soccer Club EESC 2003/2004 Girls |
0406-012G8U-1048 | Terrence Mashoro |
| A4 | Jackson Soccer Club JSC 2003 Girls |
0609-01IG8U-9878 | Stanislav Jamscek |
| A5 | Knoxville Reds Football Club - 01 Knoxville Reds 03 Santos |
0290-01D1G8U-0016 | Derrick Long |
| A6 | ALBION SC Nashville (Formerly Southern Premier Soccer) Southern Premier Soccer - 03 Girls |
0540-02D2G8U-0024 | Jeff Dial |
| A7 | Tennessee United Soccer Club TUSC 2003 Girls Red |
0424-012G8U-0029 | Andrew Fish |